A New Method in Staining Bacteria on Microscope Slides
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Lipid staining for the electron microscope: a new method.
Tissues fixed in osmium tetroxide or in combined osmium and glutaraldehyde (Hinde), embedded in Spurr's medium, cut at 0-5-I mum and mounted in Farrants' gum medium containing ethyl gallate, show good staining of lipid-contaning structures (droplets of triglyceride, membranes, mitochondria, etc.) in the light microscope. Such preparations show moderate contrast in the electron microscope withou...
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' Supported by grant no. E-2379 from the National Institutes of Health, U. S. Public Health Service. Since then, the Jones-Krueger method has been further simplified by placing a large number of slides on moistened blotters in flat trays which are then covered with glass sheets to retain moisture. Using trays 33.7 cm long, 23.5 cm wide, 1.3 cm high, the authors have routinely placed 32 slides p...
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Two methods of teaching first-year mcdto the students. Both groups were evaluical students peripheral blood morpholated by a quiz consisting of actual blood ogy were compared. A group of 27 smears. Although the mean learning time students learned using a self-teaching per student for the color slide group was audiovisual method of 35-mm color slides significantly shorter than that for the accom...
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Fixed chromosome preparations subjected to treatments such as pH 9 (I) or trypsin hydrolysis (2) and stained with Giemsa's demonstrate similar banding patterns irrespective of the pretreatments used. It has been shown that this phenomenon is the result of modifications of DNA-protein and protein-protein associations which alter dye interaction (3-5). It appears that only Giemsa's or related sta...
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ژورنال
عنوان ژورنال: Nature
سال: 1953
ISSN: 0028-0836,1476-4687
DOI: 10.1038/1711030a0